human cd26  (Creative BioMart)

Journal: JID Innovations

Article Title: Human keratinocytes exhibit limited potential for SARS-CoV-2 infection despite ACE2 and mature cathepsin L expression

doi: 10.1016/j.xjidi.2025.100447

Figure Lengend Snippet: Differential ACE2, TMPRSS2, NRP1, CTSL, CD147, AXL, and DPP4 protein expression. Whole-cell lysates of the indicated cells were analyzed by western blot using the following antibodies: ACE2 (mAb clone AC384), TMPRSS2 (mAb clone S20014A), NRP1 (mAb clone 14H4), CTSL (mAb clone 33/1), CD147 (mAb clone HIM6), AXL (polyclonal goat IgG, AF154), and DPP4 (polyclonal goat IgG, AF1180). ( a ) Representative immunoblots from 2 to 4 independent experiments. ( b ) Densitometric quantification normalized to actin, expressed as mean ± SEM from 2 to 4 independent experiments. CTSL, cathepsin L.

Article Snippet: Primary antibodies included anti-ACE2, clone AC384 (AdipoGen Life Science/Coger, Paris, France); anti-TMPRSS2, clone S20014A; anti-NRP1, clone 14H4; anti-CD147, clone HIM6; anti–keratin 10, rabbit polyclonal Poly19054 (BioLegend); anti-CTSL, clone 33/1 (eBioscience, Thermo Fisher Scientific); anti-AXL, polyclonal goat IgG AF154; and anti-DPP4, polyclonal goat IgG AF1180 (R&D Systems, Bio-Techne SAS, Noyal Châtillon, France).

Techniques: Expressing, Western Blot

Journal: Nature Communications

Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease

doi: 10.1038/s41467-026-68958-4

Figure Lengend Snippet: a Experimental design. Blood samples were collected at 7:00 am ± 15 min ( n = 10) or 11:30 am ± 15 min ( n = 10) from recipients with malignant hematological diseases in complete remission prior to cord blood infusion. b , c Serum concentrations of cytokines before cord blood infusion. Normalized z score ( b ) and absolute concentrations ( c ) of cytokines between 7:00 am and 11:30 am. The P values are two-sided and reported as exact values. d Absolute concentrations of soluble CD26/DPPIV in serum post-MAC. Blood samples were collected from recipients at 7:00 am ± 15 min ( n = 19), 11:30 am ± 15 min ( n = 19), or 4:00 pm ± 15 min ( n = 29). e – i Associations between serum soluble CD26/DPPIV (sCD26/DPPIV) levels and inflammatory cytokines before cord blood infusion on day 0. Pearson’s correlation coefficient ( r ) was calculated for the relationships between serum DPPIV levels and IL-1Ra ( e ) ( r = 0.476, P = 0.034), IL-1β ( f ) ( r = 0.450, P = 0.046), LIF ( g ) ( r = 0.517, P = 0.020), IL-18 ( h ) ( r = −0.811, P < 0.001) and IL-1α ( i ) ( r = 0.006, P = 0.741) concentrations. The P values are two-sided and reported as exact values.The data are presented as the means ± SEMs and were analyzed by unpaired t test followed by Bonferroni-Dunn correction ( c ), one-way ANOVA with Bonferroni multiple comparisons ( d ) and Pearson’s correlation ( e – i ). Source data are provided as a Source Data file.

Article Snippet: Cultures were maintained for 96 h in the presence of either recombinant human CD26/DPPIV protein (1000 ng/mL; 11244-SE, R&D Systems) or Sitagliptin phosphate monohydrate (200 μg/mL; S4002, Selleck).

Techniques:

Journal: Nature Communications

Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease

doi: 10.1038/s41467-026-68958-4

Figure Lengend Snippet: a , b Representative immunofluorescence images ( a ) and quantification ( b ) of CD26/DPPIV levels in intestinal epithelial cells from a healthy population at different times ( n = 3 patients per group) ( P < 0.001). Intestinal samples were collected at 10 am (CT10), 2 pm (CT14), and 6 pm (CT18). Scale bars, 50 μm. c Bmal1 and DPP4 expression in primary mouse epidermal cells postsynchronization in vitro ( n = 3 biological replicates, each from an independent primary culture prepared and synchronized on separate days). The Gapdh gene was used as an internal control. The relative expression levels were computed via the 2 −ΔΔCT method. CT6 is double plotted. d The concentration of CD26/DPPIV in the supernatant of primary mouse epidermal cells postsynchronization in vitro ( n = 3 biological replicates, each from an independent primary culture prepared and synchronized on separate days) ( P < 0.001). CT6 is double plotted. e Diurnal mRNA expression of DPP4 in mouse epidermal cells. Bmal1 ΔEC , epithelium cell-specific Bmal1 knockout mice. Statistics were obtained from a publicly available dataset ( GSE190035 ), with values representing the log2-transformed mean RPKM from 4 biological replicates per time point. ZT0 is double plotted.The data are presented as the means ± SEMs and were analyzed by one-way ANOVA with Bonferroni multiple comparisons ( b , d ) and JTK_Cycle ( c , e ). Source data are provided as a Source Data file.

Article Snippet: Cultures were maintained for 96 h in the presence of either recombinant human CD26/DPPIV protein (1000 ng/mL; 11244-SE, R&D Systems) or Sitagliptin phosphate monohydrate (200 μg/mL; S4002, Selleck).

Techniques: Immunofluorescence, Expressing, In Vitro, Control, Concentration Assay, Knock-Out, Transformation Assay

Journal: Nature Communications

Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease

doi: 10.1038/s41467-026-68958-4

Figure Lengend Snippet: PBMCs from healthy donors were cultured with PHA-L (control group), PHA-L plus CD26/DPPIV (sCD26 group), or PHA-L plus Sitagliptin (DPPIV inhibitor, DPPIVi group) for 72 h. a Experimental design. PBMCs from healthy donors were cultured with PHA-L (control group), PHA-L plus CD26/DPPIV (sCD26 group), or PHA-L plus Sitagliptin (DPPIV inhibitor, DPPIVi group) for 72 h. b , c Representative plots (left) and quantified percentages (right) of CD86 + ( b ) and CD80 + ( c ) CD14 + cells after 72 h of PBMCs coculture in different groups (pooled data from 8 healthy donors across four independent experiments). d – i Representative plots (left) and quantified percentages (right) of Ki-67 + , IFNγ + , and CD38 + cells among CD4 + T cells ( d , f , h ) and CD8 + T cells ( e , g , i ) after 72 h of PBMCs coculture in different groups (pooled data from 6 healthy donors across three independent experiments).The data are presented as the means ± SEMs and were analyzed by one-way ANOVA with the Bonferroni correction for multiple comparisons ( b – i ). All P values are two-sided and reported as exact values unless <0.001. Source data are provided as a Source Data file.

Article Snippet: Cultures were maintained for 96 h in the presence of either recombinant human CD26/DPPIV protein (1000 ng/mL; 11244-SE, R&D Systems) or Sitagliptin phosphate monohydrate (200 μg/mL; S4002, Selleck).

Techniques: Cell Culture, Control